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Article information
2005 , Volume 10, ¹ 3, p.3-22
Chasovskikh V.V., Frumin L.L., Peltek S.E.
A "hydrodynamic" model of long polymer chain motion in pulsed field
We present a "hydrodynamic" model describing motion of the polymeric chains that accounts for the effect of tension in the chain due to the elastic forces. The model extends the diffusion reptation theory developed for the description of long polymer chain dynamics during pulse gel-electrophoresis of DNA fragments. On the basis of statistical approach a thermodynamic equation of state for the chain segment in a gel pore is derived in case of a freely jointed chain. A balance between electric forces, gradient of elastic forces and forces of friction for a piece of chain in a gel pore, is described by one nonlinear equation of diffusion type. Extensive numerical calculations which were carried out on a cluster of 11 computers confirmed that the suggested model of "one-dimensional hydrodynamics" is capable to describe complex three-dimensional behavior of long DNA chains during pulse gel-electrophoresis. Special role of chain leakages (branching) in chain dynamics is emphasized.
[full text] Author(s):
Chasovskikh V.V.
Office: Novosibirsk State University
Address: 630090, Russia, Novosibirsk, Pirogova str., 2
E-mail: frumin@phys.nsu.ru
Frumin LeonidL.
PhD. , Associate Professor
Office: Novosibirsk State University
Address: 630090, Russia, Novosibirsk, Pirogova str., 2
Phone Office: (3832) 39 78 38
E-mail: frumin@phys.nsu.ru
Peltek S.E.
Address: Russia, Novosibirsk, Novosibirsk, Pirogova str., 2
Bibliography link:
Chasovskikh V.V., Frumin L.L., Peltek S.E. A "hydrodynamic" model of long polymer chain motion in pulsed field // Computational technologies. 2005. V. 10. ¹ 3. P. 3-22
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