Article information

1999 , Volume 4, Special issue, p.86-99

Lopatin V.N., Shepelevitch N.V., Prostakova I.V.

The mathematical modeling potential of the optical characteristics of ``soft" particles and their connection with the basic forming factors (using scanning flowing cytometry as an example)

The formation of the scattered light intensity with varying size, ellipticity, and refractive index of a homogeneous spheroid oriented by the axis of revolution along the incident light direction is studied analytically and numerically. The Wentzel-Kramers-Brillouin approximation is used for relative refractive indices ranging from 1.025 to 1.200. The inverse light scattering problem is solved in the generalized coordinates making use of the ``flight" light scattering indicatrix method. The feasibility of determining the particle size at a distance between indicatrix extrema is considered. The patterns of the formation of the radiation scattered by particles into the solid angle $\theta_0$ (integral light scattering indicatrix F) are obtained in connection with the change in the size, phase shift parameter ($\Delta$) and relative refractive index. The theoretical and numerical investigation reveals that F can be represented as a combination of two mechanisms, i.,e., Fraunhofer diffraction and geometrical optics ($\Delta \geq 3$). The feasibility of determining the relative refractive index of the studied particles has been shown.

Author(s):
Lopatin V N
Office: Institut Computing Simulation of SB RAS
Address: 660036, Russia, Krasnoyarsk, Akademgorodok

Shepelevitch NikolayV.
PhD.
Office: Institut Computing Simulation of SB RAS
Address: 660036, Russia, Krasnoyarsk, Akademgorodok
E-mail: jet@icm.krasn.ru

Prostakova I V
Office: Institut Computing Simulation of SB RAS
Address: 660036, Russia, Krasnoyarsk, Akademgorodok


Bibliography link:
Lopatin V.N., Shepelevitch N.V., Prostakova I.V. The mathematical modeling potential of the optical characteristics of ``soft" particles and their connection with the basic forming factors (using scanning flowing cytometry as an example) // Computational technologies. 1999. V. 4. Special issue. P. 86-99
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